Composite
pSP6+phoA

Part:BBa_K784030:Design

Designed by: Lior Levy   Group: iGEM12_Technion   (2012-09-17)


pSP6+RBS+Alkaline Phosphatase (pHO)+Terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1506
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1506
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1506
    Illegal XhoI site found at 18
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1506
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1506
    Illegal NgoMIV site found at 345
    Illegal NgoMIV site found at 792
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

we used XhoI and XmaI restriction sites for the ligation because these sites are not anywhere in the sequence.


Source

The promoter and terminator are from:Temme Karsten, et al. 2012. Modular control of multiple pathways using engineered orthogonal T7 polymerases. Nucleic Acids Research (advance access): 1-9. The RBS is from the parts registry,BBa_B0064 made by Endi Lab (2008) The pHOA gene is from the parts registry,BBa_J61032 made by Arkin Lab (2006)

References